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Transmute very low good quality weapon, an Eld rune, and a chipped gem to generate normal good quality weapon of the exact same kind.
When Korra afterwards journeyed across the frozen tundra towards the South Pole, Naga served as her loyal mount. During their journey, the polar bear Puppy sensed that they ended up getting followed by various dim spirits, and she or he alerted the humans with the occasion for their presence by growling from the direction with the entities.
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Each and every 6 attacks from naga and her illusions, the Naga Siren and her photographs will strike all close by units by using a damaging wave of h2o that lowers armor for 4 seconds.
m isn't limited because of the association rate constant for the development from the NagA-substrate elaborate. These effects (together with the enhanced fee of response of compound six relative to compound one) are only per the summary that NagA is restricted by both the formation or breakdown in the tetrahedral intermediate.
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cat vs. pH profile, a single ionization is noticed for both Zn- or Cd-NagA. This ionization should signify the protonation in the metallic-bound hydroxide that may be used for substrate hydrolysis or, alternatively, the final foundation that is certainly utilized to deprotonate the metal-sure water molecule just before substrate hydrolysis. The p
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a price is marginally larger for Cd-NagA than for Zn-NagA, as expected within the inclination of zinc to lessen the p
this residue is hydrogen bonded for the lone drinking water molecule (or hydroxide) which is coordinated on the metal ion sure to the Mβ-web-site (21). Thus, it is anticipated that this link naga26 residue will perform in catalysis by abstraction of the proton from water just before, or concomitant with, the attack of hydroxide about the amide bond from the substrate. Within the detection restrictions of our assay for products development, we were unable to measure any catalytic activity for both the D273N or D273A mutant.
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